TY - JOUR
T1 - Urine and serum-based ELISA using a recombinant protein and synthetic peptide for the diagnosis of tegumentary leishmaniasis
AU - Freitas, Camila S.
AU - Câmara, Raquel S.B.
AU - Lage, Daniela P.
AU - Vale, Danniele L.
AU - Silva, Ana L.
AU - Pimenta, Breno L.
AU - Ludolf, Fernanda
AU - Galvani, Nathália C.
AU - de Jesus, Marcelo M.
AU - Assis, Bárbara P.N.
AU - Chaves, Ana T.
AU - Tavares, Grasiele S.V.
AU - Tupinambás, Unaí
AU - Chávez-Fumagalli, Miguel A.
AU - Pascoal, Vanessa P.M.
AU - Eller, Marcela T.C.
AU - Rocha, Manoel O.da Costa
AU - Christodoulides, Myron
AU - Machado-de-Ávila, Ricardo A.
AU - Gonçalves, Denise U.
AU - Pereira, Isabela A.G.
AU - Coelho, Eduardo A.F.
N1 - Publisher Copyright:
© 2024 Elsevier Inc.
PY - 2025/3
Y1 - 2025/3
N2 - The diagnosis of tegumentary leishmaniasis (TL) presents problems by the variable sensitivity and specificity of the tests, and the biological samples used are also invasive. Here, ELISA experiments were performed using paired TL patient urine and serum samples in reaction against the recombinant LiHyS protein, a predicted B cell epitope and parasite antigenic extract (SLA). Two hundred and five paired samples were used, which were provided by TL patients, healthy controls and patients with Chagas disease, leprosy, malaria or HIV-infected. An urine-based ELISA showed sensitivity values of 100%, 92.1%, and 82.5%, when rLiHyS, peptide and SLA were used, respectively; and specificity of 100%, 87.6%, and 79.5%, respectively. A serum-based ELISA showed sensitivity values of 100%, 99.3%, and 81.5%, using rLiHyS, peptide and SLA, respectively, and sensitivity of 100%, 96.5%, and 72.2%, respectively. In both cases, rLiHyS presented the better performance to diagnose TL by using patient serum and urine.
AB - The diagnosis of tegumentary leishmaniasis (TL) presents problems by the variable sensitivity and specificity of the tests, and the biological samples used are also invasive. Here, ELISA experiments were performed using paired TL patient urine and serum samples in reaction against the recombinant LiHyS protein, a predicted B cell epitope and parasite antigenic extract (SLA). Two hundred and five paired samples were used, which were provided by TL patients, healthy controls and patients with Chagas disease, leprosy, malaria or HIV-infected. An urine-based ELISA showed sensitivity values of 100%, 92.1%, and 82.5%, when rLiHyS, peptide and SLA were used, respectively; and specificity of 100%, 87.6%, and 79.5%, respectively. A serum-based ELISA showed sensitivity values of 100%, 99.3%, and 81.5%, using rLiHyS, peptide and SLA, respectively, and sensitivity of 100%, 96.5%, and 72.2%, respectively. In both cases, rLiHyS presented the better performance to diagnose TL by using patient serum and urine.
KW - Diagnosis
KW - ELISA
KW - Recombinant protein
KW - Serum
KW - Tegumentary leishmaniasis
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=85210707751&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2024.116631
DO - 10.1016/j.diagmicrobio.2024.116631
M3 - Article
AN - SCOPUS:85210707751
SN - 0732-8893
VL - 111
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 3
M1 - 116631
ER -