TY - JOUR
T1 - Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis
AU - Gonçalves, Ana Alice Maia
AU - Ribeiro, Anna Julia
AU - Resende, Carlos Ananias Aparecido
AU - Couto, Carolina Alves Petit
AU - Gandra, Isadora Braga
AU - dos Santos Barcelos, Isabelle Caroline
AU - da Silva, Jonatas Oliveira
AU - Machado, Juliana Martins
AU - Silva, Kamila Alves
AU - Silva, Líria Souza
AU - dos Santos, Michelli
AU - da Silva Lopes, Lucas
AU - de Faria, Mariana Teixeira
AU - Pereira, Sabrina Paula
AU - Xavier, Sandra Rodrigues
AU - Aragão, Matheus Motta
AU - Candida-Puma, Mayron Antonio
AU - de Oliveira, Izadora Cristina Moreira
AU - Souza, Amanda Araujo
AU - Nogueira, Lais Moreira
AU - da Paz, Mariana Campos
AU - Coelho, Eduardo Antônio Ferraz
AU - Giunchetti, Rodolfo Cordeiro
AU - de Freitas, Sonia Maria
AU - Chávez-Fumagalli, Miguel Angel
AU - Nagem, Ronaldo Alves Pinto
AU - Galdino, Alexsandro Sobreira
N1 - Publisher Copyright:
© The Author(s) 2024.
PY - 2024/12
Y1 - 2024/12
N2 - Recombinant multiepitope proteins (RMPs) are a promising alternative for application in diagnostic tests and, given their wide application in the most diverse diseases, this review article aims to survey the use of these antigens for diagnosis, as well as discuss the main points surrounding these antigens. RMPs usually consisting of linear, immunodominant, and phylogenetically conserved epitopes, has been applied in the experimental diagnosis of various human and animal diseases, such as leishmaniasis, brucellosis, cysticercosis, Chagas disease, hepatitis, leptospirosis, leprosy, filariasis, schistosomiasis, dengue, and COVID-19. The synthetic genes for these epitopes are joined to code a single RMP, either with spacers or fused, with different biochemical properties. The epitopes’ high density within the RMPs contributes to a high degree of sensitivity and specificity. The RMPs can also sidestep the need for multiple peptide synthesis or multiple recombinant proteins, reducing costs and enhancing the standardization conditions for immunoassays. Methods such as bioinformatics and circular dichroism have been widely applied in the development of new RMPs, helping to guide their construction and better understand their structure. Several RMPs have been expressed, mainly using the Escherichia coli expression system, highlighting the importance of these cells in the biotechnological field. In fact, technological advances in this area, offering a wide range of different strains to be used, make these cells the most widely used expression platform. RMPs have been experimentally used to diagnose a broad range of illnesses in the laboratory, suggesting they could also be useful for accurate diagnoses commercially. On this point, the RMP method offers a tempting substitute for the production of promising antigens used to assemble commercial diagnostic kits.
AB - Recombinant multiepitope proteins (RMPs) are a promising alternative for application in diagnostic tests and, given their wide application in the most diverse diseases, this review article aims to survey the use of these antigens for diagnosis, as well as discuss the main points surrounding these antigens. RMPs usually consisting of linear, immunodominant, and phylogenetically conserved epitopes, has been applied in the experimental diagnosis of various human and animal diseases, such as leishmaniasis, brucellosis, cysticercosis, Chagas disease, hepatitis, leptospirosis, leprosy, filariasis, schistosomiasis, dengue, and COVID-19. The synthetic genes for these epitopes are joined to code a single RMP, either with spacers or fused, with different biochemical properties. The epitopes’ high density within the RMPs contributes to a high degree of sensitivity and specificity. The RMPs can also sidestep the need for multiple peptide synthesis or multiple recombinant proteins, reducing costs and enhancing the standardization conditions for immunoassays. Methods such as bioinformatics and circular dichroism have been widely applied in the development of new RMPs, helping to guide their construction and better understand their structure. Several RMPs have been expressed, mainly using the Escherichia coli expression system, highlighting the importance of these cells in the biotechnological field. In fact, technological advances in this area, offering a wide range of different strains to be used, make these cells the most widely used expression platform. RMPs have been experimentally used to diagnose a broad range of illnesses in the laboratory, suggesting they could also be useful for accurate diagnoses commercially. On this point, the RMP method offers a tempting substitute for the production of promising antigens used to assemble commercial diagnostic kits.
KW - Bioinformatics
KW - Biophysical analysis
KW - Escherichia coli
KW - Immunodiagnosis
KW - Recombinant multiepitope proteins
UR - http://www.scopus.com/inward/record.url?scp=85194000875&partnerID=8YFLogxK
U2 - 10.1186/s12934-024-02418-w
DO - 10.1186/s12934-024-02418-w
M3 - Review article
C2 - 38778337
AN - SCOPUS:85194000875
SN - 1475-2859
VL - 23
JO - Microbial Cell Factories
JF - Microbial Cell Factories
IS - 1
M1 - 145
ER -