TY - JOUR
T1 - Performance of Leishmania braziliensis enolase protein for the serodiagnosis of canine and human visceral leishmaniosis
AU - Duarte, Mariana Costa
AU - Lage, Daniela Pagliara
AU - Martins, Vívian Tamietti
AU - Costa, Lourena Emanuele
AU - Salles, Beatriz Cristina Silveira
AU - Carvalho, Ana Maria Ravena Severino
AU - de Oliveira Santos, Thaís Teodoro
AU - Dias, Daniel Silva
AU - Ribeiro, Patrícia Aparecida Fernandes
AU - Chávez-Fumagalli, Miguel Angel
AU - Machado-de-Ávila, Ricardo Andrez
AU - Roatt, Bruno Mendes
AU - Menezes-Souza, Daniel
AU - de Magalhães-Soares, Danielle Ferreira
AU - Ferraz Coelho, Eduardo Antonio
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/4/30
Y1 - 2017/4/30
N2 - In the present study, Leishmania braziliensis enolase was cloned and the recombinant protein (rEnolase) was evaluated for the serodiagnosis of canine and human visceral leishmaniosis (VL). For the canine VL diagnosis, this study examined serum samples of Leishmania infantum-infected dogs, from non-infected animals living in endemic or non-endemic areas of leishmaniosis, as well as those from Leish-Tec®-vaccinated dogs and Trypanosoma cruzi or Ehrlichia canis experimentally infected animals. For the human VL diagnosis, this study analyzed serum samples from VL patients, from non-infected subjects living in endemic or non-endemic areas of leishmaniosis, as well as those from T. cruzi-infected patients. In the results, an indirect ELISA method using rEnolase showed diagnostic sensitivity and specificity values of 100% and 98.57%, respectively, for canine VL serodiagnosis, and of 100% and 97.87%, respectively, for human VL diagnosis. These results showed rEnolase with an improved diagnostic performance when compared to the recombinant A2 protein, the crude soluble Leishmania antigenic preparation, and the recombinant K39-based immunochromatographic test. In conclusion, preliminary results suggest that the detection of antibodies against rEnolase improves the serodiagnosis of human and canine visceral leishmaniosis.
AB - In the present study, Leishmania braziliensis enolase was cloned and the recombinant protein (rEnolase) was evaluated for the serodiagnosis of canine and human visceral leishmaniosis (VL). For the canine VL diagnosis, this study examined serum samples of Leishmania infantum-infected dogs, from non-infected animals living in endemic or non-endemic areas of leishmaniosis, as well as those from Leish-Tec®-vaccinated dogs and Trypanosoma cruzi or Ehrlichia canis experimentally infected animals. For the human VL diagnosis, this study analyzed serum samples from VL patients, from non-infected subjects living in endemic or non-endemic areas of leishmaniosis, as well as those from T. cruzi-infected patients. In the results, an indirect ELISA method using rEnolase showed diagnostic sensitivity and specificity values of 100% and 98.57%, respectively, for canine VL serodiagnosis, and of 100% and 97.87%, respectively, for human VL diagnosis. These results showed rEnolase with an improved diagnostic performance when compared to the recombinant A2 protein, the crude soluble Leishmania antigenic preparation, and the recombinant K39-based immunochromatographic test. In conclusion, preliminary results suggest that the detection of antibodies against rEnolase improves the serodiagnosis of human and canine visceral leishmaniosis.
KW - Dogs
KW - Enolase
KW - Humans
KW - Leishmaniosis
KW - Recombinant protein
KW - Serodiagnosis
UR - http://www.scopus.com/inward/record.url?scp=85016594981&partnerID=8YFLogxK
U2 - 10.1016/j.vetpar.2017.03.024
DO - 10.1016/j.vetpar.2017.03.024
M3 - Article
C2 - 28385540
AN - SCOPUS:85016594981
SN - 0304-4017
VL - 238
SP - 77
EP - 81
JO - Veterinary Parasitology
JF - Veterinary Parasitology
ER -