Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection

Patrícia A.F. Ribeiro, Daniel S. Dias, Daniela P. Lage, Vívian T. Martins, Lourena E. Costa, Thaís T.O. Santos, Fernanda F. Ramos, Grasiele S.V. Tavares, Débora V.C. Mendonça, Fernanda Ludolf, Dawidson A. Gomes, Michele A. Rodrigues, Miguel A. Chávez-Fumagalli, Eduardo S. Silva, Alexsandro S. Galdino, Mariana C. Duarte, Bruno M. Roatt, Daniel Menezes-Souza, Antonio L. Teixeira, Eduardo A.F. Coelho

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

16 Citas (Scopus)

Resumen

Vaccination is one the most important strategies for the prevention of visceral leishmaniasis (VL). In the current study, a new Leishmania hypothetical protein, LiHyP, which was previously showed as antigenic in an immunoproteomic search in canine VL, was evaluated regarding its immunogenicity and protective efficacy against Leishmania infantum infection. The effects of the immunization using LiHyP were evaluated when administered as a DNA plasmid (DNA LiHyP) or recombinant protein (rLiHyP) associated with saponin. The immunity elicited by both vaccination regimens reduced the parasitism in liver, spleen, bone marrow and draining lymph nodes, being associated with high levels of IFN-γ IL-12, GM-CSF, and specific IgG2a antibody, besides low production of IL-4, IL-10, and protein and parasite-specific IgG1 antibodies. CD4 + T cells contributed more significantly to IFN-γ production in the rLiHyP/saponin group, while CD8 + T cells were more important in the production of this cytokine in the DNA LiHyP group. In addition, increased IFN-γ secretion, along with low levels of IL-10, were found when PBMCs from treated VL subject and healthy individuals were stimulated with the recombinant protein. In conclusion, when administered either as a DNA plasmid or recombinant protein, LiHyP can direct the immune response towards a Th1 immune profile, protecting animals against L. infantum infection; therefore, it can be seen as a promising immunogen against human VL.

Idioma originalInglés
Páginas (desde-hasta)108-118
Número de páginas11
PublicaciónMolecular Immunology
Volumen106
DOI
EstadoPublicada - feb. 2019
Publicado de forma externa

Huella

Profundice en los temas de investigación de 'Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection'. En conjunto forman una huella única.

Citar esto