Evaluation of two recombinant Leishmania proteins identified by an immunoproteomic approach as tools for the serodiagnosis of canine visceral and human tegumentary leishmaniasis

Eduardo Antonio Ferraz Coelho, Lourena Emanuele Costa, Daniela Pagliara Lage, Vívian Tamietti Martins, Esther Garde, Nathália Cristina de Jesus Pereira, Eliane Gonçalves Paiva Lopes, Luiz Felipe Nunes Menezes Borges, Mariana Costa Duarte, Daniel Menezes-Souza, Danielle Ferreira de Magalhães-Soares, Miguel Angel Chávez-Fumagalli, Manuel Soto, Carlos Alberto Pereira Tavares

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

24 Citas (Scopus)

Resumen

Serological diagnostic tests for canine and human leishmaniasis present problems related with their sensitivity and/or specificity. Recently, an immunoproteomic approach performed with Leishmania infantum proteins identified new parasite antigens. In the present study, the diagnostic properties of two of these proteins, cytochrome c oxidase and IgE-dependent histamine-releasing factor, were evaluated for the serodiagnosis of canine visceral (CVL) and human tegumentary (HTL) leishmaniasis. For the CVL diagnosis, sera samples from non-infected dogs living in an endemic or non-endemic area of leishmaniasis, sera from asymptomatic or symptomatic visceral leishmaniasis (VL) dogs, from Leish-Tec®-vaccinated dogs, and sera from animals experimentally infected by Trypanosoma cruzi or Ehrlichia canis were used. For the HTL diagnosis, sera from non-infected subjects living in an endemic area of leishmaniasis, sera from active cutaneous or mucosal leishmaniasis patients, as well as those from T. cruzi-infected patients were employed. ELISA assays using the recombinant proteins showed both sensitivity and specificity values of 100% for the serodiagnosis of both forms of disease, with high positive and negative predictive values, showing better diagnostic properties than the parasite recombinant A2 protein or a soluble Leishmania antigen extract. In this context, the two new recombinant proteins could be considered to be used in the serodiagnosis of CVL and HTL.

Idioma originalInglés
Páginas (desde-hasta)63-71
Número de páginas9
PublicaciónVeterinary Parasitology
Volumen215
DOI
EstadoPublicada - 15 ene. 2016
Publicado de forma externa

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