TY - JOUR
T1 - Evaluation of Leishmania infantum pyridoxal kinase protein for the diagnosis of human and canine visceral leishmaniasis
AU - Oliveira-da-Silva, João A.
AU - Machado, Amanda S.
AU - Ramos, Fernanda F.
AU - Tavares, Grasiele S.V.
AU - Lage, Daniela P.
AU - Ludolf, Fernanda
AU - Steiner, Bethina T.
AU - Reis, Thiago A.R.
AU - Santos, Thaís T.O.
AU - Costa, Lourena E.
AU - Martins, Vívian T.
AU - Galvani, Nathália C.
AU - Chaves, Ana T.
AU - Oliveira, Jamil S.
AU - Chávez-Fumagalli, Miguel A.
AU - de Magalhães-Soares, Danielle F.
AU - Duarte, Mariana C.
AU - Menezes-Souza, Daniel
AU - Silveira, Julia A.G.
AU - Moreira, Ricardo L.F.
AU - Machado-de-Ávila, Ricardo A.
AU - Tupinambás, Unaí
AU - Gonçalves, Denise U.
AU - Coelho, Eduardo A.F.
N1 - Publisher Copyright:
© 2020 European Federation of Immunological Societies
PY - 2020/4
Y1 - 2020/4
N2 - Visceral leishmaniasis (VL) is a highly neglected disease that is present in several countries worldwide. Present-day treatments against this disease are unsuitable, mainly due to the toxicity and/or high cost of drugs. In addition, the development of vaccines is still insufficient. In this scenario, a prompt VL diagnosis was deemed necessary, although sensitivity and/or specificity values of the tests have been. In this context, new antigenic candidates should be identified to be employed in a more precise diagnosis of canine and human VL. In this light, the present study evaluated the diagnostic efficacy of the Leishmania infantum pyridoxal kinase (PK) protein, applied in its recombinant version (rPK). In addition, one specific B-cell epitope derived of the PK sequence was predicted, synthetized, and evaluated as diagnostic marker. Results in ELISA tests showed that the antigens were highly sensitive to VL identification in dogs and human sera, presenting a low reactivity with VL-related disease samples. The recombinant A2 (rA2) protein and L. infantum antigenic preparation (SLA), used as controls, also proved to be highly sensitive in detecting symptomatic cases, although a low sensitivity was found when asymptomatic sera were analyzed. High cross-reactivity was also found when these antigens were evaluated against VL-related disease samples. The post-therapeutic serological follow-up showed that anti-rPK and anti-peptide IgG antibody levels decreased in significant levels after treatment. By contrast, the presence of high levels of the anti-rA2 and anti-SLA antibodies was still detected after therapy. In conclusion, rPK and its specific B-cell epitope should be considered for future studies as a diagnostic marker for canine and human VL.
AB - Visceral leishmaniasis (VL) is a highly neglected disease that is present in several countries worldwide. Present-day treatments against this disease are unsuitable, mainly due to the toxicity and/or high cost of drugs. In addition, the development of vaccines is still insufficient. In this scenario, a prompt VL diagnosis was deemed necessary, although sensitivity and/or specificity values of the tests have been. In this context, new antigenic candidates should be identified to be employed in a more precise diagnosis of canine and human VL. In this light, the present study evaluated the diagnostic efficacy of the Leishmania infantum pyridoxal kinase (PK) protein, applied in its recombinant version (rPK). In addition, one specific B-cell epitope derived of the PK sequence was predicted, synthetized, and evaluated as diagnostic marker. Results in ELISA tests showed that the antigens were highly sensitive to VL identification in dogs and human sera, presenting a low reactivity with VL-related disease samples. The recombinant A2 (rA2) protein and L. infantum antigenic preparation (SLA), used as controls, also proved to be highly sensitive in detecting symptomatic cases, although a low sensitivity was found when asymptomatic sera were analyzed. High cross-reactivity was also found when these antigens were evaluated against VL-related disease samples. The post-therapeutic serological follow-up showed that anti-rPK and anti-peptide IgG antibody levels decreased in significant levels after treatment. By contrast, the presence of high levels of the anti-rA2 and anti-SLA antibodies was still detected after therapy. In conclusion, rPK and its specific B-cell epitope should be considered for future studies as a diagnostic marker for canine and human VL.
KW - Diagnosis
KW - Leishmania infantum
KW - Pyridoxal kinase
KW - Recombinant proteins
KW - Synthetic peptides
KW - Visceral leishmaniasis
UR - http://www.scopus.com/inward/record.url?scp=85078132565&partnerID=8YFLogxK
U2 - 10.1016/j.imlet.2020.01.005
DO - 10.1016/j.imlet.2020.01.005
M3 - Article
C2 - 31981576
AN - SCOPUS:85078132565
SN - 0165-2478
VL - 220
SP - 11
EP - 20
JO - Immunology Letters
JF - Immunology Letters
ER -