Characterization of Pulmonary Bacteriobiota in Critically Ill Patients in Southern Peru through Next-Generation Sequencing Technology

Katherine Quispe-Medina, Jani Pacheco-Aranibar, Angel Mamani-Ruelas, Carlos Gamez-Bernabé, Rosemary Zapana-Begazo, Ivan Paz-Aliaga, Jose Villanueva Salas, Julio C. Bernabé-Ortiz

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Sequence variation in the 16S gene is widely used to characterize diverse microbial communities. This was the first pilot study carried out in our region where the pulmonary microbiota of critically ill patients was investigated and analyzed, with the aim of finding a specific profile for these patients that can be used as a diagnostic marker. An study of critical patients mechanically ventilated for non-respiratory indications, in a polyvalent intensive care unit, was carried out; samplee were extracted by endotracheal aspiration and subsequently the microbiota was characterized through Next-Generation Sequencing Technology (NGS). The predominant phyla among the critically ill patients were Proteobacteria, Firmicutes and Bacteroidata. In the surviving patients group, the predominant phyla were Proteobacteria, Bacteroidata and Firmicutes, in the group of deceased patients thy were Firmicutes, Proteobacteria, and Bacteroidata. We found a decrease in commensal bacteria in deceased patients and a progressive increase in in-hospital germs.

Idioma originalInglés
Páginas (desde-hasta)10041-10055
Número de páginas15
PublicaciónCurrent Issues in Molecular Biology
Volumen45
N.º12
DOI
EstadoPublicada - dic. 2023

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