TY - JOUR
T1 - Antileishmanial activity of standardized fractions of Stryphnodendron obovatum (Barbatimão) extract and constituent compounds
AU - Ribeiro, Tatiana G.
AU - Nascimento, André M.
AU - Henriques, Bárbara O.
AU - Chávez-Fumagalli, Miguel A.
AU - Franca, Juçara R.
AU - Duarte, Mariana C.
AU - Lage, Paula S.
AU - Andrade, Pedro H.R.
AU - Lage, Daniela P.
AU - Rodrigues, Lívia B.
AU - Costa, Lourena E.
AU - Martins, Vivian T.
AU - Faraco, André A.G.
AU - Coelho, Eduardo A.F.
AU - Castilho, Rachel O.
N1 - Publisher Copyright:
© 2015 Published by Elsevier Ireland Ltd.
PY - 2015/5/13
Y1 - 2015/5/13
N2 - Ethnopharmacological relevance Stryphnodendron obovatum Benth. is a Brazilian tree used to treat skin ulceration, promote wound healing, and inhibit the growth of protozoa, including Trypanosoma and Leishmania species. Bioguided fractionation of the ethanol extract of S. obovatum stem bark was performed, and antileishmanial and antioxidant activities of the standardized fractions were analyzed. Materials and methods Stationary-phase Leishmania amazonensis promastigotes, murine macrophages, and human red blood cells (RBCs) were exposed to plant extract, standardized fractions or isolated compounds for 48 h at 37 °C to evaluate their antiparasitic activity and cytotoxicity. The 2,2-diphenyl-1-picryl-hidrazyl assay was used to evaluate antioxidant activity. Results The S. obovatum extract and fractions showed antileishmanial and antioxidant activity; however, the organic fraction (OF) showed the best efficacy. We identified gallic acid, gallocatechin, epigallocatechin, catechin, and epigallocatechin gallate in the OF fraction. These compounds effectively inhibited L. amazonensis activity, with gallic acid, gallocatechin, and epigallocatechin gallate showing the highest selectivity. Furthermore, the evaluated compounds had no significant effect on murine macrophages and human RBCs. Conclusions The compounds present in the S. obovatum plant bark ethanol extract may provide an alternative therapeutic approach for L. amazonensis treatment.
AB - Ethnopharmacological relevance Stryphnodendron obovatum Benth. is a Brazilian tree used to treat skin ulceration, promote wound healing, and inhibit the growth of protozoa, including Trypanosoma and Leishmania species. Bioguided fractionation of the ethanol extract of S. obovatum stem bark was performed, and antileishmanial and antioxidant activities of the standardized fractions were analyzed. Materials and methods Stationary-phase Leishmania amazonensis promastigotes, murine macrophages, and human red blood cells (RBCs) were exposed to plant extract, standardized fractions or isolated compounds for 48 h at 37 °C to evaluate their antiparasitic activity and cytotoxicity. The 2,2-diphenyl-1-picryl-hidrazyl assay was used to evaluate antioxidant activity. Results The S. obovatum extract and fractions showed antileishmanial and antioxidant activity; however, the organic fraction (OF) showed the best efficacy. We identified gallic acid, gallocatechin, epigallocatechin, catechin, and epigallocatechin gallate in the OF fraction. These compounds effectively inhibited L. amazonensis activity, with gallic acid, gallocatechin, and epigallocatechin gallate showing the highest selectivity. Furthermore, the evaluated compounds had no significant effect on murine macrophages and human RBCs. Conclusions The compounds present in the S. obovatum plant bark ethanol extract may provide an alternative therapeutic approach for L. amazonensis treatment.
KW - Antioxidant
KW - Cerrado
KW - Gallic acid
KW - Gallocatechin
KW - Leishmania amazonensis
KW - Tannins
UR - http://www.scopus.com/inward/record.url?scp=84925436629&partnerID=8YFLogxK
U2 - 10.1016/j.jep.2015.02.047
DO - 10.1016/j.jep.2015.02.047
M3 - Article
C2 - 25732835
AN - SCOPUS:84925436629
SN - 0378-8741
VL - 165
SP - 238
EP - 242
JO - Journal of Ethnopharmacology
JF - Journal of Ethnopharmacology
ER -