TY - JOUR
T1 - A conserved Leishmania hypothetical protein evaluated for the serodiagnosis of canine and human visceral and tegumentary leishmaniasis, as well as a serological marker for the posttreatment patient follow-up
AU - Ribeiro, Patrícia A.F.
AU - Dias, Daniel S.
AU - Lage, Daniela P.
AU - Costa, Lourena E.
AU - Salles, Beatriz C.S.
AU - Steiner, Bethina T.
AU - Ramos, Fernanda F.
AU - Lima, Mariana P.
AU - Santos, Thaís T.O.
AU - Chaves, Ana T.
AU - Chávez-Fumagalli, Miguel A.
AU - Fujiwara, Ricardo T.
AU - Bueno, Lílian L.
AU - Caligiorne, Rachel B.
AU - de Magalhães-Soares, Danielle F.
AU - Silveira, Julia A.G.
AU - Machado-de-Ávila, Ricardo A.
AU - Gonçalves, Denise U.
AU - Coelho, Eduardo A.F.
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/11
Y1 - 2018/11
N2 - In the present study, a conserved Leishmania hypothetical protein, LiHyE, was evaluated for the serodiagnosis of leishmaniasis. Results showed that it presented high sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) to serologically identify visceral leishmaniasis (VL) dogs when 40 positive sera and 95 cross-reactive samples were used. rLiHyE also showed the best results of sensitivity, specificity, PPV, and NPV to identify tegumentary leishmaniasis (TL) and VL patients when 45 leishmaniasis patients’ sera and 90 cross-reactive samples were used. Results were better in comparison to those obtained when rA2 or Leishmania antigenic extract was employed as controls. The posttreatment follow-up showed that rLiHyE-specific antibodies declined significantly after the end of treatments, and a predominance of the IgG2 subclass was found in comparison to IgG1 levels in both TL and VL patients. In conclusion, rLiHyE can be considered a candidate for the serodiagnosis of canine and human leishmaniasis.
AB - In the present study, a conserved Leishmania hypothetical protein, LiHyE, was evaluated for the serodiagnosis of leishmaniasis. Results showed that it presented high sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) to serologically identify visceral leishmaniasis (VL) dogs when 40 positive sera and 95 cross-reactive samples were used. rLiHyE also showed the best results of sensitivity, specificity, PPV, and NPV to identify tegumentary leishmaniasis (TL) and VL patients when 45 leishmaniasis patients’ sera and 90 cross-reactive samples were used. Results were better in comparison to those obtained when rA2 or Leishmania antigenic extract was employed as controls. The posttreatment follow-up showed that rLiHyE-specific antibodies declined significantly after the end of treatments, and a predominance of the IgG2 subclass was found in comparison to IgG1 levels in both TL and VL patients. In conclusion, rLiHyE can be considered a candidate for the serodiagnosis of canine and human leishmaniasis.
KW - ELISA
KW - Follow-up
KW - Hypothetical proteins
KW - Serodiagnosis
KW - Tegumentary leishmaniasis
KW - Visceral leishmaniasis
UR - http://www.scopus.com/inward/record.url?scp=85048877116&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2018.05.026
DO - 10.1016/j.diagmicrobio.2018.05.026
M3 - Article
C2 - 29941364
AN - SCOPUS:85048877116
SN - 0732-8893
VL - 92
SP - 196
EP - 203
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 3
ER -