TY - JOUR
T1 - 5-HT induces temporomandibular joint nociception in rats through the local release of inflammatory mediators and activation of local β adrenoceptors
AU - Oliveira-Fusaro, Maria Cláudia G.
AU - Clemente-Napimoga, Juliana Trindade
AU - Teixeira, Juliana Maia
AU - Torres-Chávez, Karla Elena
AU - Parada, Carlos Amílcar
AU - Tambeli, Cláudia Herrera
N1 - Funding Information:
We thank Carlos Alberto Feciliano for the excellent technical assistance. This work has been supported by CNPq and by FAPESP , Brazil.
PY - 2012/9
Y1 - 2012/9
N2 - The 5-hydroxytryptamine (serotonin, 5-HT) is an important inflammatory mediator found in high levels in the synovial fluid of the temporomandibular joint (TMJ) of patients with inflammatory pain. In this study, we used the nociceptive behavior responses, measured as flinching the head and rubbing the orofacial region, as a nociceptive assay. We demonstrated that the local blockade of the 5-HT3 receptor and β1 or β2-adrenoceptors, the depletion of norepinephrine in the sympathetic terminals and the local inhibition of cyclooxygenase significantly reduced 5-HT-induced TMJ nociception. These results demonstrated that 5-HT induces nociception in the TMJ region by the activation of β1 and β2 adrenoceptors located in the TMJ region and local release of sympathetic amines and prostaglandins. Therefore, the high levels of 5-HT in the synovial fluid of patients with TMJ inflammatory pain may contribute to TMJ pain by similar mechanisms.
AB - The 5-hydroxytryptamine (serotonin, 5-HT) is an important inflammatory mediator found in high levels in the synovial fluid of the temporomandibular joint (TMJ) of patients with inflammatory pain. In this study, we used the nociceptive behavior responses, measured as flinching the head and rubbing the orofacial region, as a nociceptive assay. We demonstrated that the local blockade of the 5-HT3 receptor and β1 or β2-adrenoceptors, the depletion of norepinephrine in the sympathetic terminals and the local inhibition of cyclooxygenase significantly reduced 5-HT-induced TMJ nociception. These results demonstrated that 5-HT induces nociception in the TMJ region by the activation of β1 and β2 adrenoceptors located in the TMJ region and local release of sympathetic amines and prostaglandins. Therefore, the high levels of 5-HT in the synovial fluid of patients with TMJ inflammatory pain may contribute to TMJ pain by similar mechanisms.
KW - Nociception
KW - Norepinephrine
KW - Prostaglandins
KW - Serotonin (5HT)
KW - Temporomandibular joint
UR - http://www.scopus.com/inward/record.url?scp=84863084108&partnerID=8YFLogxK
U2 - 10.1016/j.pbb.2012.06.005
DO - 10.1016/j.pbb.2012.06.005
M3 - Article
C2 - 22683622
AN - SCOPUS:84863084108
SN - 0091-3057
VL - 102
SP - 458
EP - 464
JO - Pharmacology Biochemistry and Behavior
JF - Pharmacology Biochemistry and Behavior
IS - 3
ER -