Urine and serum-based ELISA using a recombinant protein and synthetic peptide for the diagnosis of tegumentary leishmaniasis

Camila S. Freitas, Raquel S.B. Câmara, Daniela P. Lage, Danniele L. Vale, Ana L. Silva, Breno L. Pimenta, Fernanda Ludolf, Nathália C. Galvani, Marcelo M. de Jesus, Bárbara P.N. Assis, Ana T. Chaves, Grasiele S.V. Tavares, Unaí Tupinambás, Miguel A. Chávez-Fumagalli, Vanessa P.M. Pascoal, Marcela T.C. Eller, Manoel O.da Costa Rocha, Myron Christodoulides, Ricardo A. Machado-de-Ávila, Denise U. GonçalvesIsabela A.G. Pereira, Eduardo A.F. Coelho

Research output: Contribution to journalArticlepeer-review

Abstract

The diagnosis of tegumentary leishmaniasis (TL) presents problems by the variable sensitivity and specificity of the tests, and the biological samples used are also invasive. Here, ELISA experiments were performed using paired TL patient urine and serum samples in reaction against the recombinant LiHyS protein, a predicted B cell epitope and parasite antigenic extract (SLA). Two hundred and five paired samples were used, which were provided by TL patients, healthy controls and patients with Chagas disease, leprosy, malaria or HIV-infected. An urine-based ELISA showed sensitivity values of 100%, 92.1%, and 82.5%, when rLiHyS, peptide and SLA were used, respectively; and specificity of 100%, 87.6%, and 79.5%, respectively. A serum-based ELISA showed sensitivity values of 100%, 99.3%, and 81.5%, using rLiHyS, peptide and SLA, respectively, and sensitivity of 100%, 96.5%, and 72.2%, respectively. In both cases, rLiHyS presented the better performance to diagnose TL by using patient serum and urine.

Original languageEnglish
Article number116631
JournalDiagnostic Microbiology and Infectious Disease
Volume111
Issue number3
DOIs
StatePublished - Mar 2025

Keywords

  • Diagnosis
  • ELISA
  • Recombinant protein
  • Serum
  • Tegumentary leishmaniasis
  • Urine

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