TY - JOUR
T1 - Theranostic applications of phage display to control leishmaniasis
T2 - Selection of biomarkers for serodiagnostics, vaccination, and immunotherapy
AU - Coelho, Eduardo Antonio Ferraz
AU - Chávez-Fumagalli, Miguel Angel
AU - Costa, Lourena Emanuele
AU - Tavares, Carlos Alberto Pereira
AU - Soto, Manuel
AU - Goulart, Luiz Ricardo
N1 - Publisher Copyright:
© 2015, Sociedade Brasileira de Medicina Tropical. All rights reserved.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Phage display is a high-throughput subtractive proteomic technology used for the generation and screening of large peptide and antibody libraries. It is based on the selection of phage-fused surface-exposed peptides that recognize specific ligands and demonstrate desired functionality for diagnostic and therapeutic purposes. Phage display has provided unmatched tools for controlling viral, bacterial, fungal, and parasitic infections, and allowed identification of new therapeutic targets to treat cancer, metabolic diseases, and other chronic conditions. This review presents recent advancements in serodiagnostics and prevention of leishmaniasis -an important tropical parasitic disease- achieved using phage display for the identification of novel antigens with improved sensitivity and specificity. Our focus is on theranostics of visceral leishmaniasis with the aim to develop biomarker candidates exhibiting both diagnostic and therapeutic potential to fight this important, yet neglected, tropical disease.
AB - Phage display is a high-throughput subtractive proteomic technology used for the generation and screening of large peptide and antibody libraries. It is based on the selection of phage-fused surface-exposed peptides that recognize specific ligands and demonstrate desired functionality for diagnostic and therapeutic purposes. Phage display has provided unmatched tools for controlling viral, bacterial, fungal, and parasitic infections, and allowed identification of new therapeutic targets to treat cancer, metabolic diseases, and other chronic conditions. This review presents recent advancements in serodiagnostics and prevention of leishmaniasis -an important tropical parasitic disease- achieved using phage display for the identification of novel antigens with improved sensitivity and specificity. Our focus is on theranostics of visceral leishmaniasis with the aim to develop biomarker candidates exhibiting both diagnostic and therapeutic potential to fight this important, yet neglected, tropical disease.
KW - Mimotopes
KW - Phage display
KW - Serodiagnosis
KW - Vaccine
KW - Visceral leishmaniasis
UR - http://www.scopus.com/inward/record.url?scp=84940383336&partnerID=8YFLogxK
U2 - 10.1590/0037-8682-0096-2015
DO - 10.1590/0037-8682-0096-2015
M3 - Review article
C2 - 26312925
AN - SCOPUS:84940383336
SN - 0037-8682
VL - 48
SP - 370
EP - 379
JO - Revista da Sociedade Brasileira de Medicina Tropical
JF - Revista da Sociedade Brasileira de Medicina Tropical
IS - 4
ER -