TY - JOUR
T1 - Strychnos pseudoquina and its purified compounds present an effective in vitro antileishmanial activity
AU - Lage, Paula Sousa
AU - De Andrade, Pedro Henrique Rocha
AU - Lopes, Amanda De Santana
AU - Chávez Fumagalli, Miguel Angel
AU - Valadares, Diogo Garcia
AU - Duarte, Mariana Costa
AU - Pagliara Lage, Daniela
AU - Costa, Lourena Emanuele
AU - Martins, Vivian Tamietti
AU - Ribeiro, Tatiana Gomes
AU - Filho, José Dias De Souza
AU - Tavares, Carlos Alberto Pereira
AU - Pádua, Rodrigo Maia De
AU - Leite, João Paulo Viana
AU - Coelho, Eduardo Antonio Ferraz
PY - 2013
Y1 - 2013
N2 - The development of new and cost-effective alternative therapeutic strategies to treat leishmaniasis has become a high priority. In the present study, the antileishmanial activity of Strychnos pseudoquina St. Hil. was investigated and pure compounds that presented this biological effect were isolated. An ethyl acetate extract was prepared, and it proved to be effective against Leishmania amazonensis. A bioactivity-guided fractionation was performed, and two flavonoids were identified, quercetin 3-O-methyl ether and strychnobiflavone, which presented an effective antileishmanial activity against L. amazonensis, and studies were extended to establish their minimum inhibitory concentrations (IC50), their leishmanicidal effects on the intra-macrophage Leishmania stage, as well as their cytotoxic effects on murine macrophages (CC50), and in O+ human red blood cells. The data presented in this study showed the potential of an ethyl acetate extract of S. pseudoquina, as well as two flavonoids purified from it, which can be used as a therapeutic alternative on its own, or in association with other drugs, to treat disease evoked by L. amazonensis.
AB - The development of new and cost-effective alternative therapeutic strategies to treat leishmaniasis has become a high priority. In the present study, the antileishmanial activity of Strychnos pseudoquina St. Hil. was investigated and pure compounds that presented this biological effect were isolated. An ethyl acetate extract was prepared, and it proved to be effective against Leishmania amazonensis. A bioactivity-guided fractionation was performed, and two flavonoids were identified, quercetin 3-O-methyl ether and strychnobiflavone, which presented an effective antileishmanial activity against L. amazonensis, and studies were extended to establish their minimum inhibitory concentrations (IC50), their leishmanicidal effects on the intra-macrophage Leishmania stage, as well as their cytotoxic effects on murine macrophages (CC50), and in O+ human red blood cells. The data presented in this study showed the potential of an ethyl acetate extract of S. pseudoquina, as well as two flavonoids purified from it, which can be used as a therapeutic alternative on its own, or in association with other drugs, to treat disease evoked by L. amazonensis.
UR - http://www.scopus.com/inward/record.url?scp=84886685366&partnerID=8YFLogxK
U2 - 10.1155/2013/304354
DO - 10.1155/2013/304354
M3 - Article
AN - SCOPUS:84886685366
SN - 1741-427X
VL - 2013
JO - Evidence-based Complementary and Alternative Medicine
JF - Evidence-based Complementary and Alternative Medicine
M1 - 304354
ER -