TY - JOUR
T1 - Serodiagnosis of canine leishmaniasis using a novel recombinant chimeric protein constructed with distinct B-cell epitopes from antigenic Leishmania infantum proteins
AU - Vale, Danniele L.
AU - Lage, Daniela P.
AU - Machado, Amanda S.
AU - Freitas, Camila S.
AU - de Oliveira, Daysiane
AU - Galvani, Nathália C.
AU - Fernandes, Bruna B.
AU - Luiz, Gabriel P.
AU - Oliveira, Jamil S.
AU - Oliveira-da-Silva, João A.
AU - Ramos, Fernanda F.
AU - Santos, Thaís T.O.
AU - Siqueira, Williane F.
AU - Alves, Livia A.
AU - Chávez-Fumagalli, Miguel A.
AU - de Magalhães-Soares, Danielle F.
AU - Silveira, Julia A.G.
AU - Bueno, Lílian L.
AU - Fujiwara, Ricardo T.
AU - Machado-de-Ávila, Ricardo A.
AU - Martins, Vívian T.
AU - Coelho, Eduardo A.F.
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/8
Y1 - 2021/8
N2 - Visceral leishmaniasis (VL) is an important public health problem in the world, and control measures are insufficient to avoid the spread of this neglected disease. Dogs are important domestic reservoirs of Leishmania parasites in countries where VL is a zoonosis, representing a major source of infection between sand fly vectors and humans. In this context, a precise diagnosis of canine leishmaniasis (CanL) could help to reduce the number of human cases. Distinct approaches for the diagnosis of CanL have used recombinant proteins in serological assays. However, variable results of the antigens have been found, mainly to diagnosis asymptomatic cases. The present study used bioinformatics to select specific B-cell epitopes of four Leishmania infantum proteins, which had previously been proven to be antigenic in VL, aiming to produce a novel chimeric protein and to evaluate it for the diagnosis of CanL. Seven B-cell epitopes were identified and used to construct the chimera, which was analyzed in a recombinant format through an ELISA assay against a canine serological panel. A soluble Leishmania antigenic extract (SLA) was used as an antigen control. Results showed 100 % sensitivity and specificity for chimera, while when using SLA the values were 26.0 % and 96.4 %, respectively. The performance of chimera was compared with a commercial kit using asymptomatic and symptomatic dog sera, and the data showed that no false-negative result was found when the recombinant protein was used. However, when using the commercial kit, 40.0 % and 16.0 % of the false-negative results were found, respectively. In conclusion, the recombinant chimera showed an antigenic potential to be evaluated in new studies against a larger serological panel for the diagnosis of CanL.
AB - Visceral leishmaniasis (VL) is an important public health problem in the world, and control measures are insufficient to avoid the spread of this neglected disease. Dogs are important domestic reservoirs of Leishmania parasites in countries where VL is a zoonosis, representing a major source of infection between sand fly vectors and humans. In this context, a precise diagnosis of canine leishmaniasis (CanL) could help to reduce the number of human cases. Distinct approaches for the diagnosis of CanL have used recombinant proteins in serological assays. However, variable results of the antigens have been found, mainly to diagnosis asymptomatic cases. The present study used bioinformatics to select specific B-cell epitopes of four Leishmania infantum proteins, which had previously been proven to be antigenic in VL, aiming to produce a novel chimeric protein and to evaluate it for the diagnosis of CanL. Seven B-cell epitopes were identified and used to construct the chimera, which was analyzed in a recombinant format through an ELISA assay against a canine serological panel. A soluble Leishmania antigenic extract (SLA) was used as an antigen control. Results showed 100 % sensitivity and specificity for chimera, while when using SLA the values were 26.0 % and 96.4 %, respectively. The performance of chimera was compared with a commercial kit using asymptomatic and symptomatic dog sera, and the data showed that no false-negative result was found when the recombinant protein was used. However, when using the commercial kit, 40.0 % and 16.0 % of the false-negative results were found, respectively. In conclusion, the recombinant chimera showed an antigenic potential to be evaluated in new studies against a larger serological panel for the diagnosis of CanL.
KW - Commercial kit
KW - Dogs
KW - ELISA
KW - Recombinant chimera
KW - Serodiagnosis
KW - Visceral leishmaniasis
UR - http://www.scopus.com/inward/record.url?scp=85109431032&partnerID=8YFLogxK
U2 - 10.1016/j.vetpar.2021.109513
DO - 10.1016/j.vetpar.2021.109513
M3 - Article
C2 - 34225189
AN - SCOPUS:85109431032
SN - 0304-4017
VL - 296
JO - Veterinary Parasitology
JF - Veterinary Parasitology
M1 - 109513
ER -