TY - JOUR
T1 - Proof of Concept of a Novel Multiepitope Recombinant Protein for the Serodiagnosis of Patients with Chagas Disease
AU - Machado, Juliana Martins
AU - Pereira, Isabela Amorim Gonçalves
AU - Maia, Ana Clara Gontijo
AU - Francisco, Mariana Ferraz Chaves
AU - Nogueira, Lais Moreira
AU - Gandra, Isadora Braga
AU - Ribeiro, Anna Julia
AU - Silva, Kamila Alves
AU - Resende, Carlos Ananias Aparecido
AU - da Silva, Jonatas Oliveira
AU - dos Santos, Michelli
AU - Gonçalves, Ana Alice Maia
AU - Tavares, Grasiele de Sousa Vieira
AU - Chávez-Fumagalli, Miguel Angel
AU - Campos-da-Paz, Mariana
AU - Giunchetti, Rodolfo Cordeiro
AU - Rocha, Manoel Otávio da Costa
AU - Chaves, Ana Thereza
AU - Coelho, Eduardo Antônio Ferraz
AU - Galdino, Alexsandro Sobreira
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/2
Y1 - 2023/2
N2 - Chagas disease remains a neglected disease that is considered to be a public health problem. The early diagnosis of cases is important to improve the prognosis of infected patients and prevent transmission. Serological tests are the method of choice for diagnosis. However, two serological tests are currently recommended to confirm positive cases. In this sense, more sensitive and specific serological tests need to be developed to overcome these current diagnosis problems. This study aimed to develop a new recombinant multiepitope protein for the diagnosis of Chagas disease, hereafter named rTC. The rTC was constructed based on amino acid sequences from different combinations of Trypanosoma cruzi antigens in the same polypeptide and tested using an enzyme-linked immunosorbent assay (ELISA) to detect different types of Chagas disease. rTC was able to discriminate between indeterminate (IND) and cardiac (CARD) cases and cross-reactive diseases, as well as healthy samples, with 98.28% sensitivity and 96.67% specificity, respectively. These data suggest that rTC has the potential to be tested in future studies against a larger serological panel for the diagnosis of Chagas disease.
AB - Chagas disease remains a neglected disease that is considered to be a public health problem. The early diagnosis of cases is important to improve the prognosis of infected patients and prevent transmission. Serological tests are the method of choice for diagnosis. However, two serological tests are currently recommended to confirm positive cases. In this sense, more sensitive and specific serological tests need to be developed to overcome these current diagnosis problems. This study aimed to develop a new recombinant multiepitope protein for the diagnosis of Chagas disease, hereafter named rTC. The rTC was constructed based on amino acid sequences from different combinations of Trypanosoma cruzi antigens in the same polypeptide and tested using an enzyme-linked immunosorbent assay (ELISA) to detect different types of Chagas disease. rTC was able to discriminate between indeterminate (IND) and cardiac (CARD) cases and cross-reactive diseases, as well as healthy samples, with 98.28% sensitivity and 96.67% specificity, respectively. These data suggest that rTC has the potential to be tested in future studies against a larger serological panel for the diagnosis of Chagas disease.
KW - Chagas disease
KW - immunodiagnosis
KW - multiepitope
KW - recombinant protein
UR - http://www.scopus.com/inward/record.url?scp=85148707934&partnerID=8YFLogxK
U2 - 10.3390/pathogens12020312
DO - 10.3390/pathogens12020312
M3 - Article
AN - SCOPUS:85148707934
SN - 2076-0817
VL - 12
JO - Pathogens
JF - Pathogens
IS - 2
M1 - 312
ER -