Probing the efficacy of a heterologous Leishmania/L. Viannia braziliensis recombinant enolase as a candidate vaccine to restrict the development of L. infantum in BALB/c mice

Thaís T.O. Santos, Vívian T. Martins, Daniela P. Lage, Lourena E. Costa, Beatriz C.S. Salles, Ana M.R.S. Carvalho, Daniel S. Dias, Patrícia A.F. Ribeiro, Miguel A. Chávez-Fumagalli, Ricardo A. Machado-de-Ávila, Bruno M. Roatt, Danielle F. de Magalhães-Soares, Daniel Menezes-Souza, Eduardo A.F. Coelho, Mariana C. Duarte

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

In the present study, the Leishmania braziliensis enolase protein was evaluated as a vaccine candidate against visceral leishmaniasis (VL). The DNA sequence was cloned and the recombinant protein (rEnolase) was evaluated as a vaccine, associated with saponin, as an immune adjuvant. The protective efficacy of the rEnolase plus saponin combination was investigated in BALB/c mice against Leishmania infantum infection. The results revealed that the vaccine induced higher levels of IFN-γ, IL-12, and GM-CSF when a capture ELISA and flow cytometry were performed, as well as an antileishmanial nitrite production after using in vitro stimulation with rEnolase and an antigenic Leishmania preparation. The vaccinated animals, when compared to the control groups, showed a lower parasite burden in the liver, spleen, bone marrow, and paws’ draining lymph nodes when both a limiting dilution technique and RT-PCR assay were performed. In addition, these mice showed low levels of antileishmanial IL-4, IL-10, and anti-Leishmania IgG1 isotype antibodies. Partial protection was associated with IFN-γ production, which was mainly mediated by CD4+ T cells. In conclusion, the present study's data showed that the L. braziliensis enolase protein could be considered a vaccine candidate that offers heterologous protection against VL.

Original languageEnglish
Pages (from-to)8-16
Number of pages9
JournalActa Tropica
Volume171
DOIs
StatePublished - 1 Jul 2017
Externally publishedYes

Keywords

  • Enolase
  • Immune response
  • Leishmania braziliensis
  • Leishmania infantum
  • Vaccine

Fingerprint

Dive into the research topics of 'Probing the efficacy of a heterologous Leishmania/L. Viannia braziliensis recombinant enolase as a candidate vaccine to restrict the development of L. infantum in BALB/c mice'. Together they form a unique fingerprint.

Cite this