Low-melanin containing pullulan production from sugarcane bagasse hydrolysate by Aureobasidium pullulans in fermentations assisted by light-emitting diode

Ruly Terán Hilares, Camila Ayres Orsi, Muhammad Ajaz Ahmed, Paulo Franco Marcelino, Carlos Renato Menegatti, Silvio Silvério da Silva, Júlio César dos Santos

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Pullulan is a polymer produced by Aureobasidium pullulans and the main bottleneck for its industrial production is the presence of melanin pigment. In this study, light-emitting diodes (LEDs) of different wavelengths were used to assist the fermentation process aiming to produce low-melanin containing pullulan by wild strain of A. pullulans LB83 with different carbon sources. Under white light using glucose-based medium, 11.75 g.L−1of pullulan with high melanin content (45.70 UA540nm.g−1) was obtained, this production improved in process assisted by blue LED light, that resulted in 15.77 g.L−1of pullulan with reduced content of melanin (4.46 UA540nm.g−1). By using sugarcane bagasse (SCB) hydrolysate as carbon source, similar concentration of pullulan (about 20 g.L−1) was achieved using white and blue LED lights, with lower melanin contents in last. Use of LED light was found as a promising approach to assist biotechnological process for low-melanin containing pullulan production.

Original languageEnglish
Pages (from-to)76-81
Number of pages6
JournalBioresource Technology
Volume230
DOIs
StatePublished - 2017
Externally publishedYes

Keywords

  • Enzymatic hydrolysate
  • Exopolysaccharide (EPS)
  • Light-emitting diode
  • Melanin pigment
  • Pullulan
  • Sugarcane bagasse

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