Leishmania infantum mimotopes and a phage–ELISA assay as tools for a sensitive and specific serodiagnosis of human visceral leishmaniasis

Beatriz C.S. Salles, Lourena E. Costa, Patrícia T. Alves, Ana C.S. Dias, Emília R. Vaz, Daniel Menezes-Souza, Fernanda F. Ramos, Mariana C. Duarte, Bruno M. Roatt, Miguel A. Chávez-Fumagalli, Carlos A.P. Tavares, Denise U. Gonçalves, Regina L. Rocha, Luiz R. Goulart, Eduardo A.F. Coelho

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Serological methods used to diagnose visceral leishmaniasis (VL) are considered minimally invasive, but they present problems related with their sensitivity and/or specificity. In this study, a subtractive selection using the phage display technology against antibodies from healthy subjects living in endemic and non-endemic areas of disease, as well as from Chagas disease patients and those developing active VL, was developed. The aim of this study was to select bacteriophage-fused epitopes to be used in the serodiagnosis of human VL. Eight phage clones were selected after the bio-panning rounds, and their reactivity was evaluated in a phage-ELISA assay against a human serological panel. A wild-type clone and the recombinant K39-based immunochromatographic test were used as controls. In the results, it was shown that all clones showed an excellent performance to serologically identify VL patients, demonstrating the feasibility of the isolated phages for developing a specific and sensitive serodiagnosis of human VL.

Original languageEnglish
Pages (from-to)219-225
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume87
Issue number3
DOIs
StatePublished - 1 Mar 2017
Externally publishedYes

Keywords

  • Mimotopes
  • Phage display
  • Phage–ELISA
  • Serodiagnosis
  • Specificity
  • Visceral leishmaniasis

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