TY - JOUR
T1 - In vitro microbiological and drug release of silver/ibuprofen loaded wound dressing designed for the treatment of chronically infected painful wounds
AU - Mogrovejo-Valdivia, Alejandra
AU - Maton, Mickael
AU - Garcia-Fernandez, Maria Jose
AU - Tabary, Nicolas
AU - Chai, Feng
AU - Neut, Christel
AU - Martel, Bernard
AU - Blanchemain, Nicolas
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/7
Y1 - 2021/7
N2 - This study consisted of developing a dressing loaded with silver (Ag) and ibuprofen (IBU) that provides a dual therapy, antibacterial and antalgic, intended for infected painful wounds. There-fore, non-woven polyethyleneterephtalate (PET) textiles nonwovens were pre-treated by cyclodextrin crosslinked with citric acid by a pad/dry/cure process. Then, textiles were impregnated in silver so-lution followed by a thermal treatment and were then coated by Layer-by-Layer (L-b-L) deposition of a polyelectrolyte multilayer (PEM) system consisting of anionic water-soluble poly(betacyclodextrin citrate) (PCD) and cationic chitosan. Finally, ibuprofen lysinate (IBU-L) was loaded on the PEM coating. We demonstrated the complexation of IBU with native βCD and PCD by phase solubility diagram and1 H NMR. PEM system allowed complete IBU-L release in 6 h in PBS pH 7.4 batch (USP IV). On the other hand, microbiological tests demonstrated that loaded silver induced bacterial reduction of 4 Log10 against S. aureus and E. coli and tests revealed that ibuprofen lysinate loading did not interfere with the antibacterial properties of the dressing.
AB - This study consisted of developing a dressing loaded with silver (Ag) and ibuprofen (IBU) that provides a dual therapy, antibacterial and antalgic, intended for infected painful wounds. There-fore, non-woven polyethyleneterephtalate (PET) textiles nonwovens were pre-treated by cyclodextrin crosslinked with citric acid by a pad/dry/cure process. Then, textiles were impregnated in silver so-lution followed by a thermal treatment and were then coated by Layer-by-Layer (L-b-L) deposition of a polyelectrolyte multilayer (PEM) system consisting of anionic water-soluble poly(betacyclodextrin citrate) (PCD) and cationic chitosan. Finally, ibuprofen lysinate (IBU-L) was loaded on the PEM coating. We demonstrated the complexation of IBU with native βCD and PCD by phase solubility diagram and1 H NMR. PEM system allowed complete IBU-L release in 6 h in PBS pH 7.4 batch (USP IV). On the other hand, microbiological tests demonstrated that loaded silver induced bacterial reduction of 4 Log10 against S. aureus and E. coli and tests revealed that ibuprofen lysinate loading did not interfere with the antibacterial properties of the dressing.
KW - Anti-inflammatory
KW - Antibacterial
KW - Cyclodextrins
KW - Drug delivery system
KW - Layer by layer
KW - Wound dressing
UR - http://www.scopus.com/inward/record.url?scp=85110279038&partnerID=8YFLogxK
U2 - 10.3390/antibiotics10070805
DO - 10.3390/antibiotics10070805
M3 - Article
AN - SCOPUS:85110279038
SN - 2079-6382
VL - 10
JO - Antibiotics
JF - Antibiotics
IS - 7
M1 - 805
ER -