TY - JOUR
T1 - Evaluation of two recombinant Leishmania proteins identified by an immunoproteomic approach as tools for the serodiagnosis of canine visceral and human tegumentary leishmaniasis
AU - Coelho, Eduardo Antonio Ferraz
AU - Costa, Lourena Emanuele
AU - Lage, Daniela Pagliara
AU - Martins, Vívian Tamietti
AU - Garde, Esther
AU - de Jesus Pereira, Nathália Cristina
AU - Lopes, Eliane Gonçalves Paiva
AU - Borges, Luiz Felipe Nunes Menezes
AU - Duarte, Mariana Costa
AU - Menezes-Souza, Daniel
AU - de Magalhães-Soares, Danielle Ferreira
AU - Chávez-Fumagalli, Miguel Angel
AU - Soto, Manuel
AU - Tavares, Carlos Alberto Pereira
N1 - Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2016/1/15
Y1 - 2016/1/15
N2 - Serological diagnostic tests for canine and human leishmaniasis present problems related with their sensitivity and/or specificity. Recently, an immunoproteomic approach performed with Leishmania infantum proteins identified new parasite antigens. In the present study, the diagnostic properties of two of these proteins, cytochrome c oxidase and IgE-dependent histamine-releasing factor, were evaluated for the serodiagnosis of canine visceral (CVL) and human tegumentary (HTL) leishmaniasis. For the CVL diagnosis, sera samples from non-infected dogs living in an endemic or non-endemic area of leishmaniasis, sera from asymptomatic or symptomatic visceral leishmaniasis (VL) dogs, from Leish-Tec®-vaccinated dogs, and sera from animals experimentally infected by Trypanosoma cruzi or Ehrlichia canis were used. For the HTL diagnosis, sera from non-infected subjects living in an endemic area of leishmaniasis, sera from active cutaneous or mucosal leishmaniasis patients, as well as those from T. cruzi-infected patients were employed. ELISA assays using the recombinant proteins showed both sensitivity and specificity values of 100% for the serodiagnosis of both forms of disease, with high positive and negative predictive values, showing better diagnostic properties than the parasite recombinant A2 protein or a soluble Leishmania antigen extract. In this context, the two new recombinant proteins could be considered to be used in the serodiagnosis of CVL and HTL.
AB - Serological diagnostic tests for canine and human leishmaniasis present problems related with their sensitivity and/or specificity. Recently, an immunoproteomic approach performed with Leishmania infantum proteins identified new parasite antigens. In the present study, the diagnostic properties of two of these proteins, cytochrome c oxidase and IgE-dependent histamine-releasing factor, were evaluated for the serodiagnosis of canine visceral (CVL) and human tegumentary (HTL) leishmaniasis. For the CVL diagnosis, sera samples from non-infected dogs living in an endemic or non-endemic area of leishmaniasis, sera from asymptomatic or symptomatic visceral leishmaniasis (VL) dogs, from Leish-Tec®-vaccinated dogs, and sera from animals experimentally infected by Trypanosoma cruzi or Ehrlichia canis were used. For the HTL diagnosis, sera from non-infected subjects living in an endemic area of leishmaniasis, sera from active cutaneous or mucosal leishmaniasis patients, as well as those from T. cruzi-infected patients were employed. ELISA assays using the recombinant proteins showed both sensitivity and specificity values of 100% for the serodiagnosis of both forms of disease, with high positive and negative predictive values, showing better diagnostic properties than the parasite recombinant A2 protein or a soluble Leishmania antigen extract. In this context, the two new recombinant proteins could be considered to be used in the serodiagnosis of CVL and HTL.
KW - Canine visceral leishmaniasis
KW - Human tegumentary leishmaniasis
KW - Leishmania braziliensis
KW - Leishmania infantum
KW - Recombinant proteins
KW - Serodiagnosis
UR - http://www.scopus.com/inward/record.url?scp=84958752984&partnerID=8YFLogxK
U2 - 10.1016/j.vetpar.2015.11.006
DO - 10.1016/j.vetpar.2015.11.006
M3 - Article
C2 - 26790739
AN - SCOPUS:84958752984
SN - 0304-4017
VL - 215
SP - 63
EP - 71
JO - Veterinary Parasitology
JF - Veterinary Parasitology
ER -