Effect of the calcium silicate-based sealer removal protocols and time-point of acid etching on the dentin adhesive interface

Jéssika Mayhara Pereira Morais, Keli Regina Victorino, Wilfredo Gustavo Escalante-Otárola, Keren Cristina Fagundes Jordão-Basso, Regina Guenka Palma-Dibb, Milton Carlos Kuga

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The aim of the study was to evaluate the effects when acid etching on the dentin surface was immediately performed (I) or 7 days (D) after calcium silicate-based sealer (MTA Fillapex) removal, using 95% ethanol (E) or xylol (X). First study, 60 bovine incisor dentin specimens were impregnated with sealer and divided into six groups (n = 10): (EI), E + I; (XI), X + I; (ED), E + D; (XD), X + D, (UN), untreated and (MR), mechanical removal of sealer. Scanning electron microscopy (SEM) images (500×) were obtained from each specimen and scores assessed the sealer residues persistence. Second study, 60 specimens were similarly treated; however, the specimens were restored with composite resin after the removal protocols. Hybrid layer formation was evaluated using confocal laser microscopy (1,024×). Third study, 60 specimens were similarly obtained and subjected to micro-shear test to evaluate the effects of removal protocols on the bond strength of etch-and- rinse adhesive system to dentin. XI showed the highest persistence of sealer residues (p <.05), similar to MR (p >.05). EI showed the greatest hybrid layer extension, except in relation to UN (p <.05). XI and MR presented the lowest bond strength adhesive system to dentin (p <.05). Acid etching immediately after calcium silicate-based endodontic sealer removal using xylol presented the highest residues persistence and negatively affected the adhesive interface between dentin and etch-and-rinse adhesive system.

Original languageEnglish
Pages (from-to)914-920
Number of pages7
JournalMicroscopy Research and Technique
Volume81
Issue number8
DOIs
StatePublished - Aug 2018
Externally publishedYes

Keywords

  • dentin
  • endodontics
  • image analysis
  • scanning electron microscopy

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