TY - JOUR
T1 - Biochemical characterization and pharmacological properties of new basic PLABrTX-I isolated from Bothrops roedingeri (Roedinger's Lancehead) mertens, 1942, snake venom
AU - Gomes Heleno, Mauricio Aurelio
AU - Baldasso, Paulo Aparecido
AU - Ponce-Soto, Luis Alberto
AU - Marangoni, Sérgio
PY - 2013
Y1 - 2013
N2 - BrTX-I, a PLA was purified from Bothrops roedingeri venom after only one chromatographic step using reverse-phase HPLC on -Bondapak C-18 column. A molecular mass of 14358.69 Da was determined by MALDI-TOF mass spectrometry. Amino acid analysis showed a high content of hydrophobic and basic amino acids as well as 14 half-cysteine residues. The total amino acid sequence was obtained using SwissProt database and showed high amino acid sequence identity with other PLAfrom snake venom. The amino acid composition showed that BrTX-I has a high content of Lys, Tyr, Gly, Pro, and 14 half-Cys residues, typical of a basic PLA BrTX-I presented PLAactivity and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0, 35-45°C, and required Ca 2+. In vitro, the whole venom and BrTX-I caused a neuromuscular blockade in biventer cervicis preparations in a similar way to other Bothrops species. BrTX-I induced myonecrosis and oedema-forming activity analyzed through injection of the purified BrTX-I in mice. Since BrTX-I exerts a strong proinflammatory effect, the enzymatic phospholipid hydrolysis might be relevant for these phenomena; incrementing levels of IL-1, IL-6, and TNF α were observed at 15 min, 30 min, one, two, and six hours postinjection, respectively.
AB - BrTX-I, a PLA was purified from Bothrops roedingeri venom after only one chromatographic step using reverse-phase HPLC on -Bondapak C-18 column. A molecular mass of 14358.69 Da was determined by MALDI-TOF mass spectrometry. Amino acid analysis showed a high content of hydrophobic and basic amino acids as well as 14 half-cysteine residues. The total amino acid sequence was obtained using SwissProt database and showed high amino acid sequence identity with other PLAfrom snake venom. The amino acid composition showed that BrTX-I has a high content of Lys, Tyr, Gly, Pro, and 14 half-Cys residues, typical of a basic PLA BrTX-I presented PLAactivity and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0, 35-45°C, and required Ca 2+. In vitro, the whole venom and BrTX-I caused a neuromuscular blockade in biventer cervicis preparations in a similar way to other Bothrops species. BrTX-I induced myonecrosis and oedema-forming activity analyzed through injection of the purified BrTX-I in mice. Since BrTX-I exerts a strong proinflammatory effect, the enzymatic phospholipid hydrolysis might be relevant for these phenomena; incrementing levels of IL-1, IL-6, and TNF α were observed at 15 min, 30 min, one, two, and six hours postinjection, respectively.
UR - http://www.scopus.com/inward/record.url?scp=84874583242&partnerID=8YFLogxK
U2 - 10.1155/2013/591470
DO - 10.1155/2013/591470
M3 - Article
C2 - 23509747
AN - SCOPUS:84874583242
SN - 2314-6133
VL - 2013
JO - BioMed Research International
JF - BioMed Research International
M1 - 591470
ER -