TY - JOUR
T1 - A Mini-Review of Diagnostic Methods for the Antigen and Antibody Detection of Rocky Mountain and Brazilian Spotted Fever
AU - Silva, Kamila Alves
AU - Prado, Vanesa Borges do
AU - Silva, Rafael Rodrigues
AU - Rocha, Marcelo van Petten
AU - de Oliveira, Rafael Almeida Ribeiro
AU - Falcão, Tarumim de Jesus Rodrigues
AU - Serpa, Clara Cristina
AU - Rocha, Marina Andrade
AU - Pereira, Sabrina Paula
AU - Silva, Líria Souza
AU - Machado, Juliana Martins
AU - Machado-de-Ávila, Ricardo Andrez
AU - Fujiwara, Ricardo Toshio
AU - Chávez-Fumagalli, Miguel Angel
AU - Coelho, Eduardo Antônio Ferraz
AU - Giunchetti, Rodolfo Cordeiro
AU - Campos-da-Paz, Mariana
AU - Gonçalves, Ana Alice Maia
AU - Galdino, Alexsandro Sobreira
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/7
Y1 - 2024/7
N2 - Rocky Mountain or Brazilian spotted fever, caused by Rickettsia rickettsii, is a fulminant, seasonal, and neglected disease that occurs in focal points of North America and South America. Its rapid detection is essential for the better prognosis and survival rate of infected individuals. However, disease diagnosis still faces challenges as the accuracy of many of the available laboratory tests fluctuates. This review aimed to analyze methods for antibody or antigen detection, their gaps, and their evolution over time. A search was conducted to find all studies in the Pubmed database that described the antibody or antigen detection of R. rickettsii infections. Initially, a total of 403 articles were screened. Of these articles, only 17 fulfilled the pre-established inclusion criteria and were selected. Among the different methods applied, the IFA technique was the one most frequently found in the studies. However, it presented varied results such as a low specificity when using the indirect method. Other techniques, such as ELISA and immunohistochemistry, were also found, although in smaller numbers and with their own limitations. Although some studies showed promising results, there is a pressing need to find new techniques to develop a rapid and effective diagnosis of R. rickettssi infection.
AB - Rocky Mountain or Brazilian spotted fever, caused by Rickettsia rickettsii, is a fulminant, seasonal, and neglected disease that occurs in focal points of North America and South America. Its rapid detection is essential for the better prognosis and survival rate of infected individuals. However, disease diagnosis still faces challenges as the accuracy of many of the available laboratory tests fluctuates. This review aimed to analyze methods for antibody or antigen detection, their gaps, and their evolution over time. A search was conducted to find all studies in the Pubmed database that described the antibody or antigen detection of R. rickettsii infections. Initially, a total of 403 articles were screened. Of these articles, only 17 fulfilled the pre-established inclusion criteria and were selected. Among the different methods applied, the IFA technique was the one most frequently found in the studies. However, it presented varied results such as a low specificity when using the indirect method. Other techniques, such as ELISA and immunohistochemistry, were also found, although in smaller numbers and with their own limitations. Although some studies showed promising results, there is a pressing need to find new techniques to develop a rapid and effective diagnosis of R. rickettssi infection.
KW - Brazilian spotted fever
KW - diagnosis
KW - Rickettsia rickettsii
KW - Rocky Mountain spotted fever
UR - http://www.scopus.com/inward/record.url?scp=85199671992&partnerID=8YFLogxK
U2 - 10.3390/biomedicines12071501
DO - 10.3390/biomedicines12071501
M3 - Review article
AN - SCOPUS:85199671992
SN - 2227-9059
VL - 12
JO - Biomedicines
JF - Biomedicines
IS - 7
M1 - 1501
ER -